Reconstructing the mitochondrial protein import machinery of Chlamydomonas reinhardtii.

ABSTRACT: In Chlamydomonas reinhardtii several nucleus-encoded proteins that participate in the mitochondrial oxidative phosphorylation are targeted to the organelle by unusually long mitochondrial targeting sequences. Here, we explored the components of the mitochondrial import machinery of the green alga. We mined the algal genome, searching for yeast and plant homologs, and reconstructed the mitochondrial import machinery. All the main translocation components were identified in Chlamydomonas as well as in Arabidopsis thaliana and in the recently sequenced moss Physcomitrella patens. Some of these components appear to be duplicated, as is the case of Tim22. In contrast, several yeast components that have relatively large hydrophilic regions exposed to the cytosol or to the intermembrane space see

SUBMITTER: Figueroa-Martinez F 

PROVIDER: S-EPMC2390594 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature