Coincidence of P-insertion sites and breakpoints of deletions induced by activating P elements in Drosophila.

ABSTRACT: We isolated a set of seven deletions in the 67B region by activating a nearby P-element insertion. The structures of the deletions were characterized by cloning and sequencing. The results showed that the P-induced deletions occurred nonrandomly in the genomic sites. One breakpoint of the deletions was located precisely at the end of the starting element, i.e., at the end of the inverted terminal repeats. The other breakpoint was nearby the retained starting element and coincided with preferential P-element insertion sites that harbor transcription initiation activities. It is known that P elements induce male recombination near the starting elements, giving rise to deletions with one breakpoint precisely located at an inverted terminal repeat of the retained starting element. Database ana

SUBMITTER: Sudi J 

PROVIDER: S-EPMC2390602 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature