The dual mode of action of bistramide A entails severing of filamentous actin and covalent protein modification.

ABSTRACT: This study provides comprehensive characterization of the mode of action of bistramide A and identifies structural requirements of bistramide-based compounds that are responsible for severing actin filaments and inhibiting growth of cancer cells in vitro and in vivo. We rationally designed and assembled a series of structural analogs of the natural product, including a fluorescently labeled conjugate. We used TIRF microscopy to directly observe actin filament severing by this series of small molecules, which established that the combination of the spiroketal and the amide subunits was sufficient to enable rapid actin filament disassembly in vitro. In addition, we demonstrated that the enone subunit of bistramide A is responsible for covalent modification of the protein in vitro and in A549

SUBMITTER: Rizvi SA 

PROVIDER: S-EPMC2393769 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature