Manipulation of the mechanical properties of a virus by protein engineering.

ABSTRACT: In a previous study, we showed that the DNA molecule within a spherical virus (the minute virus of mice) plays an architectural role by anisotropically increasing the mechanical stiffness of the virus. A finite element model predicted that this mechanical reinforcement is a consequence of the interaction between crystallographically visible, short DNA patches and the inner capsid wall. We have now tested this model by using protein engineering. Selected amino acid side chains have been truncated to specifically remove major interactions between the capsid and the visible DNA patches, and the effect of the mutations on the stiffness of virus particles has been measured using atomic force microscopy. The mutations do not affect the stiffness of the empty capsid; however, they significantly r

SUBMITTER: Carrasco C 

PROVIDER: S-EPMC2393779 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature