Ontology highlight
ABSTRACT:
SUBMITTER: Thongdee M
PROVIDER: S-EPMC2394929 | biostudies-literature | 2008 May
REPOSITORIES: biostudies-literature
Thongdee Metawee M Gallagher Larry A LA Schell Mark M Dharakul Tararaj T Songsivilai Sirirurg S Manoil Colin C
Applied and environmental microbiology 20080229 10
Burkholderia pseudomallei is the causative agent of melioidosis, an overwhelming, rapidly fatal septic infection, and B. thailandensis is a closely related, less virulent species. Both organisms are naturally competent for DNA transformation, and this report describes a procedure exploiting this property for the rapid generation of marked deletion mutations by using PCR products. The method was employed to create 61 mutant strains. Several selectable elements were employed, including elements ca ...[more]