Identification of Escherichia coli YgaF as an L-2-hydroxyglutarate oxidase.

ABSTRACT: YgaF, a protein of previously unknown function in Escherichia coli, was shown to possess noncovalently bound flavin adenine dinucleotide and to exhibit L-2-hydroxyglutarate oxidase activity. The inability of anaerobic, reduced enzyme to reverse the reaction by reducing the product alpha-ketoglutaric acid is explained by the very high reduction potential (+19 mV) of the bound cofactor. The likely role of this enzyme in the cell is to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate. On the basis of the identified function, we propose that this gene be renamed lhgO.

SUBMITTER: Kalliri E 

PROVIDER: S-EPMC2395033 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature