Validation of virulence and epidemiology DNA microarray for identification and characterization of Staphylococcus aureus isolates.

ABSTRACT: The human pathogen Staphylococcus aureus is isolated and characterized using traditional culture and sensitivity methodologies that are slow and offer limited information on the organism. In contrast, DNA microarray technology can provide detailed, clinically relevant information on the isolate by detecting the presence or absence of a large number of virulence-associated genes simultaneously in a single assay. We have developed and validated a novel, cost-effective multiwell microarray for the identification and characterization of Staphylococcus aureus. The array comprises 84 gene targets, including species-specific, antibiotic resistance, toxin, and other virulence-associated genes, and is capable of examining 13 different isolates simultaneously, together with a reference control strai

SUBMITTER: Spence RP 

PROVIDER: S-EPMC2395118 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature