Proteomics analysis unravels the functional repertoire of coronavirus nonstructural protein 3.

ABSTRACT: Severe acute respiratory syndrome (SARS) coronavirus infection and growth are dependent on initiating signaling and enzyme actions upon viral entry into the host cell. Proteins packaged during virus assembly may subsequently form the first line of attack and host manipulation upon infection. A complete characterization of virion components is therefore important to understanding the dynamics of early stages of infection. Mass spectrometry and kinase profiling techniques identified nearly 200 incorporated host and viral proteins. We used published interaction data to identify hubs of connectivity with potential significance for virion formation. Surprisingly, the hub with the most potential connections was not the viral M protein but the nonstructural protein 3 (nsp3), which is one of the n

SUBMITTER: Neuman BW 

PROVIDER: S-EPMC2395186 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature