Microevolution of Dengue viruses circulating among primary school children in Kamphaeng Phet, Thailand.

ABSTRACT: To determine the extent and structure of genetic variation in dengue viruses (DENV) on a restricted spatial and temporal scale, we sequenced the E (envelope) genes of DENV-1, -2, and -3 isolates collected in 2001 from children enrolled in a prospective school-based study in Kamphaeng Phet, Thailand, and diagnosed with dengue disease. Our analysis revealed substantial viral genetic variation in both time and space, with multiple viral lineages circulating within individual schools, suggesting the frequent gene flow of DENV into this microenvironment. More-detailed analyses of DENV-2 samples revealed strong clustering of viral isolates within individual schools and evidence of more-frequent viral gene flow among schools closely related in space. Conversely, we observed little evolutionary ch

SUBMITTER: Jarman RG 

PROVIDER: S-EPMC2395201 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature