Active remodelling of the TIM23 complex during translocation of preproteins into mitochondria.

ABSTRACT: The TIM23 (translocase of the mitochondrial inner membrane) complex mediates translocation of preproteins across and their insertion into the mitochondrial inner membrane. How the translocase mediates sorting of preproteins into the two different subcompartments is poorly understood. In particular, it is not clear whether association of two operationally defined parts of the translocase, the membrane-integrated part and the import motor, depends on the activity state of the translocase. We established conditions to in vivo trap the TIM23 complex in different translocation modes. Membrane-integrated part of the complex and import motor were always found in one complex irrespective of whether an arrested preprotein was present or not. Instead, we detected different conformations of the compl

SUBMITTER: Popov-Celeketic D 

PROVIDER: S-EPMC2396396 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature