Identification of valid reference genes for the normalization of RT qPCR gene expression data in human brain tissue.

ABSTRACT:

Background

Studies of gene expression in post mortem human brain can contribute to understanding of the pathophysiology of neurodegenerative diseases, including Alzheimer's disease (AD), Parkinson's disease (PD) and dementia with Lewy bodies (DLB). Quantitative real-time PCR (RT qPCR) is often used to analyse gene expression. The validity of results obtained using RT qPCR is reliant on accurate data normalization. Reference genes are generally used to normalize RT qPCR data. Given that expression of some commonly used reference genes is altered in certain conditions, this study aimed to establish which reference genes were stably expressed in post mortem brain tissue from individuals with AD, PD or DLB.

Results

The present study investigated the expression stability of 8 can

SUBMITTER: Coulson DT 

PROVIDER: S-EPMC2396658 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature