The role of the small intestine in the development of dietary fat-induced obesity and insulin resistance in C57BL/6J mice.

ABSTRACT:

Background

Obesity and insulin resistance are two major risk factors underlying the metabolic syndrome. The development of these metabolic disorders is frequently studied, but mainly in liver, skeletal muscle, and adipose tissue. To gain more insight in the role of the small intestine in development of obesity and insulin resistance, dietary fat-induced differential gene expression was determined along the longitudinal axis of small intestines of C57BL/6J mice.

Methods

Male C57BL/6J mice were fed a low-fat or a high-fat diet that mimicked the fatty acid composition of a Western-style human diet. After 2, 4 and 8 weeks of diet intervention small intestines were isolated and divided in three equal parts. Differential gene expression was determined in mucosal scrapings using Mo

SUBMITTER: de Wit NJ 

PROVIDER: S-EPMC2396659 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature