A novel design of whole-genome microarray probes for Saccharomyces cerevisiae which minimizes cross-hybridization.

ABSTRACT:

Background

Numerous DNA microarray hybridization experiments have been performed in yeast over the last years using either synthetic oligonucleotides or PCR-amplified coding sequences as probes. The design and quality of the microarray probes are of critical importance for hybridization experiments as well as subsequent analysis of the data.

Results

We present here a novel design of Saccharomyces cerevisiae microarrays based on a refined annotation of the genome and with the aim of reducing cross-hybridization between related sequences. An effort was made to design probes of similar lengths, preferably located in the 3'-end of reading frames. The sequence of each gene was compared against the entire yeast genome and optimal sub-segments giving no predicted cross-hybridizatio

SUBMITTER: Talla E 

PROVIDER: S-EPMC239980 | biostudies-literature | 2003 Sep

REPOSITORIES: biostudies-literature