CrossSearch, a user-friendly search engine for detecting chemically cross-linked peptides in conjugated proteins.

ABSTRACT: Chemical cross-linking and high resolution MS have been integrated successfully to capture protein interactions and provide low resolution structural data for proteins that are refractive to analyses by NMR or crystallography. Despite the versatility of these combined techniques, the array of products that is generated from the cross-linking and proteolytic digestion of proteins is immense and generally requires the use of labeling strategies and/or data base search algorithms to distinguish actual cross-linked peptides from the many side products of cross-linking. Most strategies reported to date have focused on the analysis of small cross-linked protein complexes (<60 kDa) because the number of potential forms of covalently modified peptides increases dramatically with the number of pept

SUBMITTER: Nadeau OW 

PROVIDER: S-EPMC2401330 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature