Comparative evaluation of current peptide production platforms used in absolute quantification in proteomics.

ABSTRACT: Absolute quantification of peptides by mass spectrometry requires a reference, frequently using heavy isotope-coded peptides as internal standards. These peptides have traditionally been generated by chemical stepwise synthesis. Recently a new way to supply such peptides was described in which nucleotide sequences coding for the respective peptides are concatenated into a synthetic gene (QconCAT). These QconCATs are then expressed to produce a polypeptide consisting of concatenated peptides, purified, quantified by various methods, and then digested to yield the final internal standard peptides. Although both of these methods for peptide production are routinely used for absolute quantifications, there is currently no information regarding the accuracy of the quantifications made in each c

SUBMITTER: Mirzaei H 

PROVIDER: S-EPMC2401334 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature