Coupled global and targeted proteomics of human embryonic stem cells during induced differentiation.

ABSTRACT: Elucidating the complex combinations of growth factors and signaling molecules that maintain pluripotency or, alternatively, promote the controlled differentiation of human embryonic stem cells (hESCs) has important implications for the fundamental understanding of human development, devising cell replacement therapies, and cancer cell biology. hESCs are commonly grown on irradiated mouse embryonic fibroblasts (MEFs) or in conditioned medium from MEFs. These culture conditions interfere with many experimental conclusions and limit the ability to perform conclusive proteomics studies. The current investigation avoided the use of MEFs or MEF-conditioned medium for hESC culture, allowing global proteomics analysis without these confounding conditions, and elucidated neural cell-specific signa

SUBMITTER: Yocum AK 

PROVIDER: S-EPMC2401335 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature