Identification and characterization of differentially expressed cDNAs of the vector mosquito, Anopheles gambiae.

ABSTRACT: The isolation and study of Anopheles gambiae genes that are differentially expressed in development, notably in tissues associated with the maturation and transmission of the malaria parasite, is important for the elucidation of basic molecular mechanisms underlying vector-parasite interactions. We have used the differential display technique to screen for mRNAs specifically expressed in adult males, females, and midgut tissues of blood-fed and unfed females. We also screened for mRNAs specifically induced upon bacterial infection of larval stage mosquitoes. We have characterized 19 distinct cDNAs, most of which show developmentally regulated expression specificity during the mosquito life cycle. The most interesting are six new sequences that are midgut-specific in the adult, three of whi

SUBMITTER: Dimopoulos G 

PROVIDER: S-EPMC24047 | biostudies-literature | 1996 Nov

REPOSITORIES: biostudies-literature