Circadian genomics of the chick pineal gland in vitro.

ABSTRACT:

Background

Chick pinealocytes exhibit all the characteristics of a complete circadian system, comprising photoreceptive inputs, molecular clockworks and an easily measured rhythmic output, melatonin biosynthesis. These properties make the in vitro pineal a particularly useful model for exploring circadian control of gene transcription in a pacemaker tissue, as well as regulation of the transcriptome by primary inputs to the clock (both photic and noradrenergic).

Results

We used microarray analysis to investigate the expression of approximately 8000 genes within cultured pinealocytes subjected to both LD and DD. We report that a reduced subset of genes was rhythmically expressed in vitro compared to those previously published in vivo, and that gene expression rhythms were low

SUBMITTER: Karaganis SP 

PROVIDER: S-EPMC2405806 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature