PKC putative phosphorylation site Ser235 is required for MIP/AQP0 translocation to the plasma membrane.

ABSTRACT:

Purpose

To investigate the functional significance of MIP/AQP0 phosphorylation at serine(235).

Methods

MIP/AQP0 expression and cellular localization was studied in rat lens epithelia explants induced to differentiate by FGF-2. MIP wild type (WT) and MIP (S235A) mutant expression plasmids were constructed and transiently expressed in RK13 cells. Subcellular localization of endogenous MIP in differentiating lens epithelia explants or of transfected MIP expression vectors in RK13 cells was analyzed by immunofluorescence confocal microscopy.

Results

MIP/AQP0 expressed in lens epithelia explants induced to differentiate by FGF-2 localizes to the plasma membrane of elongating cells. However, MIP/AQP0 translocation to the plasma membrane was prevented by inhibiting PKC activ

SUBMITTER: Golestaneh N 

PROVIDER: S-EPMC2405813 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature