Carbon monoxide protects against ventilator-induced lung injury via PPAR-gamma and inhibition of Egr-1.

ABSTRACT:

Rationale

Ventilator-induced lung injury (VILI) leads to an unacceptably high mortality. In this regard, the antiinflammatory properties of inhaled carbon monoxide (CO) may provide a therapeutic option.

Objectives

This study explores the mechanisms of CO-dependent protection in a mouse model of VILI.

Methods

Mice were ventilated (12 ml/kg, 1-8 h) with air in the absence or presence of CO (250 ppm). Airway pressures, blood pressure, and blood gases were monitored. Lung tissue was analyzed for inflammation, injury, and gene expression. Bronchoalveolar lavage fluid was analyzed for protein, cell and neutrophil counts, and cytokines.

Measurements and main results

Mechanical ventilation caused significant lung injury reflected by increases in protein concentration,

SUBMITTER: Hoetzel A 

PROVIDER: S-EPMC2408440 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature