Expression pattern, regulation, and functions of methionine adenosyltransferase 2beta splicing variants in hepatoma cells.

ABSTRACT:

Background & aims

Methionine adenosyltransferase (MAT) catalyzes S-adenosylmethionine biosynthesis. Two genes (MAT1A and MAT2A) encode for the catalytic subunit of MAT, while a third gene (MAT2beta) encodes for a regulatory subunit that modulates the activity of MAT2A-encoded isoenzyme. We uncovered multiple splicing variants while characterizing its 5'-flanking region. The aims of our current study are to examine the expression pattern, regulation, and functions of the 2 major variants: V1 and V2.

Methods

Studies were conducted using RNA from normal human tissues, resected hepatocellular carcinoma specimens, and cell lines. Gene expression, promoter and nuclear binding activities, growth, and apoptosis were measured by routine assays.

Results

MAT2beta is expressed in

SUBMITTER: Yang H 

PROVIDER: S-EPMC2409110 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature