PIAS3 interacts with ATF1 and regulates the human ferritin H gene through an antioxidant-responsive element.

ABSTRACT: Gene transcription is coordinately regulated by the balance between activation and repression mechanisms in response to various external stimuli. Ferritin, composed of H and L subunits, is the major intracellular iron storage protein involved in iron homeostasis. We previously identified an enhancer, termed antioxidant-responsive element (ARE), in the human ferritin H gene and its respective transcriptional activators including Nrf2 and JunD. Here we found that ATF1 (activating transcription factor 1) is a transcriptional repressor of the ferritin H ARE. Subsequent yeast two-hybrid screening identified PIAS3 (protein inhibitor of activated STAT3) as an ATF1-binding protein. Further investigation of the human ferritin H ARE regulation showed that 1) PIAS3 reversed ATF1-mediated repression o

SUBMITTER: Iwasaki K 

PROVIDER: S-EPMC2409283 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature