Expression profiling in transgenic FVB/N embryonic stem cells overexpressing STAT3.

ABSTRACT:

Background

The transcription factor STAT3 is a downstream target of the LIF signalling cascade. LIF signalling or activation is sufficient to maintain embryonic stem (ES) cells in an undifferentiated and pluripotent state. To further investigate the importance of STAT3 in the establishment of ES cells we have in a first step derived stable pluripotent embryonic stem cells from transgenic FVB mice expressing a conditional tamoxifen dependent STAT3-MER fusion protein. In a second step, STAT3-MER overexpressing cells were used to identify STAT3 pathway-related genes by expression profiling in order to identify new key-players involved in maintenance of pluripotency in ES cells.

Results

Transgenic STAT3-MER blastocysts yielded pluripotent germline-competent ES cells at a high fr

SUBMITTER: Cinelli P 

PROVIDER: S-EPMC2409313 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature