Comprehensive analysis of the mouse renal cortex using two-dimensional HPLC - tandem mass spectrometry.

ABSTRACT:

Background

Proteomic methodologies increasingly have been applied to the kidney to map the renal cortical proteome and to identify global changes in renal proteins induced by diseases such as diabetes. While progress has been made in establishing a renal cortical proteome using 1-D or 2-DE and mass spectrometry, the number of proteins definitively identified by mass spectrometry has remained surprisingly small. Low coverage of the renal cortical proteome as well as our interest in diabetes-induced changes in proteins found in the renal cortex prompted us to perform an in-depth proteomic analysis of mouse renal cortical tissue.

Results

We report a large scale analysis of mouse renal cortical proteome using SCX prefractionation strategy combined with HPLC - tandem mass spectro

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC2412861 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature