Genomic content of Bordetella pertussis clinical isolates circulating in areas of intensive children vaccination.

ABSTRACT:

Background

The objective of the study was to analyse the evolution of Bordetella pertussis population and the influence of herd immunity in different areas of the world where newborns and infants are highly vaccinated.

Methodology

The analysis was performed using DNA microarray on 15 isolates, PCR on 111 isolates as well as GS-FLX sequencing technology on 3 isolates and the B. pertussis reference strain, Tohama I.

Principal findings

Our analyses demonstrate that the current circulating isolates are continuing to lose genetic material as compared to isolates circulating during the pre-vaccine era whatever the area of the world considered. The lost genetic material does not seem to be important for virulence. Our study confirms that the use of whole cell vaccines has le

SUBMITTER: Bouchez V 

PROVIDER: S-EPMC2413009 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature