Identification of active transcriptional regulatory modules by the functional assay of DNA from nucleosome-free regions.

ABSTRACT: The identification of transcriptional regulatory modules within mammalian genomes is a prerequisite to understanding the mechanisms controlling regulated gene expression. While high-throughput microarray- and sequencing-based approaches have been used to map the genomic locations of sites of nuclease hypersensitivity or target DNA sequences bound by specific protein factors, the identification of regulatory elements using functional assays, which would provide important complementary data, has been relatively rare. Here we present a method that permits the functional identification of active transcriptional regulatory modules using a simple procedure for the isolation and analysis of DNA derived from nucleosome-free regions (NFRs), the 2% of the cellular genome that contains these elements

SUBMITTER: Yaragatti M 

PROVIDER: S-EPMC2413160 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature