Isolation, characterization and molecular cloning of duplex-specific nuclease from the hepatopancreas of the Kamchatka crab.

ABSTRACT:

Background

Nucleases, which are key components of biologically diverse processes such as DNA replication, repair and recombination, antiviral defense, apoptosis and digestion, have revolutionized the field of molecular biology. Indeed many standard molecular strategies, including molecular cloning, studies of DNA-protein interactions, and analysis of nucleic acid structures, would be virtually impossible without these versatile enzymes. The discovery of nucleases with unique properties has often served as the basis for the development of modern molecular biology methods. Thus, the search for novel nucleases with potentially exploitable functions remains an important scientific undertaking.

Results

Using degenerative primers and the rapid amplification of cDNA ends (RACE) pro

SUBMITTER: Anisimova VE 

PROVIDER: S-EPMC2413221 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature