Multispacer sequence typing for Mycobacterium tuberculosis genotyping.

ABSTRACT:

Background

Genotyping methods developed to survey the transmission dynamics of Mycobacterium tuberculosis currently rely on the interpretation of restriction and amplification profiles. Multispacer sequence typing (MST) genotyping is based on the sequencing of several intergenic regions selected after complete genome sequence analysis. It has been applied to various pathogens, but not to M. tuberculosis.

Methods and findings

In M. tuberculosis, the MST approach yielded eight variable intergenic spacers which included four previously described variable number tandem repeat loci, one single nucleotide polymorphism locus and three newly evaluated spacers. Spacer sequence stability was evaluated by serial subculture. The eight spacers were sequenced in a collection of 101 M. tub

SUBMITTER: Djelouadji Z 

PROVIDER: S-EPMC2413405 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature