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Detection of active butyrate-degrading microorganisms in methanogenic sludges by RNA-based stable isotope probing.

ABSTRACT: Butyrate-degrading bacteria in four methanogenic sludges were studied by RNA-based stable isotope probing. Bacterial populations in the (13)C-labeled rRNA fractions were distinct from unlabeled fractions, and Syntrophaceae species, Tepidanaerobacter sp., and Clostridium spp. dominated. These results suggest that diverse microbes were active in butyrate degradation under methanogenic conditions.

SUBMITTER: Hatamoto M 

PROVIDER: S-EPMC2423034 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature

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Detection of active butyrate-degrading microorganisms in methanogenic sludges by RNA-based stable isotope probing.

Hatamoto Masashi M   Imachi Hiroyuki H   Yashiro Yuto Y   Ohashi Akiyoshi A   Harada Hideki H  

Applied and environmental microbiology 20080411 11

Butyrate-degrading bacteria in four methanogenic sludges were studied by RNA-based stable isotope probing. Bacterial populations in the (13)C-labeled rRNA fractions were distinct from unlabeled fractions, and Syntrophaceae species, Tepidanaerobacter sp., and Clostridium spp. dominated. These results suggest that diverse microbes were active in butyrate degradation under methanogenic conditions. ...[more]

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