Bves directly interacts with GEFT, and controls cell shape and movement through regulation of Rac1/Cdc42 activity.

ABSTRACT: Bves is an integral membrane protein with no determined function and no homology to proteins outside of the Popdc family. It is widely expressed throughout development in myriad organisms. Here, we demonstrate an interaction between Bves and guanine nucleotide exchange factor T (GEFT), a GEF for Rho-family GTPases. This interaction represents the first identification of any protein that has a direct physical interaction with any member of the Popdc family. Bves and GEFT are shown to colocalize in adult skeletal muscle. We also demonstrate that exogenous expression of Bves reduces Rac1 and Cdc42 activity levels while not affecting levels of active RhoA. Consistent with a repression of Rac1 and Cdc42 activity, we show changes in speed of cell locomotion and cell roundness also result from ex

SUBMITTER: Smith TK 

PROVIDER: S-EPMC2423412 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature