Variation in expression of HMW1 and HMW2 adhesins in invasive nontypeable Haemophilus influenzae isolates.

ABSTRACT:

Background

Among surface antigens of nontypeable Haemophilus influenzae (NTHi), the HMW1 and HMW2 proteins are the major adhesins promoting colonization of the upper respiratory tract. Since they are potential vaccine candidates, knowledge concerning variation in HMW proteins expression among clinical isolates is of great interest. In this study, expression of hmw1A and hmw2A genes was evaluated by quantitative real-time reverse transcription-PCR in 3 NTHi invasive isolates (strains 56, 72, 91) and in the prototype strain 12. Number of 7-bp repeats within the hmwA promoters and presence of HMW proteins by Western blotting were also determined.

Results

Results showed that gene transcription varied not only among different isolates but also between the hmw1A and hmw2A genes fr

SUBMITTER: Giufre M 

PROVIDER: S-EPMC2424069 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature