Time course profiling of the retinal transcriptome after optic nerve transection and optic nerve crush.

ABSTRACT:

Purpose

A time-course analysis of gene regulation in the adult rat retina after intraorbital nerve crush (IONC) and intraorbital nerve transection (IONT).

Methods

RNA was extracted from adult rat retinas undergoing either IONT or IONC at increasing times post-lesion. Affymetrix RAE230.2 arrays were hybridized and analyzed. Statistically regulated genes were annotated and functionally clustered. Arrays were validated by means of quantative reverse transcription polymerase chain reaction (qRT-PCR) on ten regulated genes at two times post-lesion. Western blotting and immunohistofluorescence for four pro-apoptotic proteins were performed on naïve and injured retinas. Finally, custom signaling maps for IONT- and IONC-induced death response were generated (MetaCore, Genego Inc.).<

SUBMITTER: Agudo M 

PROVIDER: S-EPMC2426719 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature