Plectin isoform 1b mediates mitochondrion-intermediate filament network linkage and controls organelle shape.

ABSTRACT: Plectin is a versatile intermediate filament (IF)-bound cytolinker protein with a variety of differentially spliced isoforms accounting for its multiple functions. One particular isoform, plectin 1b (P1b), remains associated with mitochondria after biochemical fractionation of fibroblasts and cells expressing exogenous P1b. Here, we determined that P1b is inserted into the outer mitochondrial membrane with the exon 1b-encoded N-terminal sequence serving as a mitochondrial targeting and anchoring signal. To study P1b-related mitochondrial functions, we generated mice that selectively lack this isoform but express all others. In primary fibroblasts and myoblasts derived from these mice, we observe a substantial elongation of mitochondrial networks, whereas other mitochondrial properties rema

SUBMITTER: Winter L 

PROVIDER: S-EPMC2426950 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature