Simultaneous gene expression profiling in human macrophages infected with Leishmania major parasites using SAGE.

ABSTRACT:

Background

Leishmania (L) are intracellular protozoan parasites that are able to survive and replicate within the harsh and potentially hostile phagolysosomal environment of mammalian mononuclear phagocytes. A complex interplay then takes place between the macrophage (MPhi) striving to eliminate the pathogen and the parasite struggling for its own survival. To investigate this host-parasite conflict at the transcriptional level, in the context of monocyte-derived human MPhis (MDM) infection by L. major metacyclic promastigotes, the quantitative technique of serial analysis of gene expression (SAGE) was used.

Results

After extracting mRNA from resting human MPhis, Leishmania-infected human MPhis and L. major parasites, three SAGE libraries were constructed and sequenced gener

SUBMITTER: Guerfali FZ 

PROVIDER: S-EPMC2430024 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature