Selection of reference genes for quantitative real-time PCR in a rat asphyxial cardiac arrest model.

ABSTRACT:

Background

Cardiac arrest, and the associated arrest of blood circulation, immediately leads to permanent brain damage because of the exhaustion of oxygen, glucose and energy resources in the brain. Most hippocampal CA1 neurons die during the first week post the insult. Molecular data concerning the recovery after resuscitation are sparse and limited to the early time period. Expression analysis of marker genes via quantitative real-time RT-PCR enables to follow up the remodeling process. However, proper validation of the applied normalization strategy is a crucial prerequisite for reliable conclusions.Therefore, the present study aimed to determine the expression stability of ten commonly used reference genes (Actb, actin, beta; B2m, beta-2 microglobulin;CypA, cyclophilin A; Gapdh

SUBMITTER: Langnaese K 

PROVIDER: S-EPMC2430208 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature