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Changes in prostate gene expression in men undergoing an intensive nutrition and lifestyle intervention.


ABSTRACT: Epidemiological and prospective studies indicate that comprehensive lifestyle changes may modify the progression of prostate cancer. However, the molecular mechanisms by which improvements in diet and lifestyle might affect the prostate microenvironment are poorly understood. We conducted a pilot study to examine changes in prostate gene expression in a unique population of men with low-risk prostate cancer who declined immediate surgery, hormonal therapy, or radiation and participated in an intensive nutrition and lifestyle intervention while undergoing careful surveillance for tumor progression. Consistent with previous studies, significant improvements in weight, abdominal obesity, blood pressure, and lipid profile were observed (all P < 0.05), and surveillance of low-risk patients was safe. Gene expression profiles were obtained from 30 participants, pairing RNA samples from control prostate needle biopsy taken before intervention to RNA from the same patient's 3-month postintervention biopsy. Quantitative real-time PCR was used to validate array observations for selected transcripts. Two-class paired analysis of global gene expression using significance analysis of microarrays detected 48 up-regulated and 453 down-regulated transcripts after the intervention. Pathway analysis identified significant modulation of biological processes that have critical roles in tumorigenesis, including protein metabolism and modification, intracellular protein traffic, and protein phosphorylation (all P < 0.05). Intensive nutrition and lifestyle changes may modulate gene expression in the prostate. Understanding the prostate molecular response to comprehensive lifestyle changes may strengthen efforts to develop effective prevention and treatment. Larger clinical trials are warranted to confirm the results of this pilot study.

SUBMITTER: Ornish D 

PROVIDER: S-EPMC2430265 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature

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Changes in prostate gene expression in men undergoing an intensive nutrition and lifestyle intervention.

Ornish Dean D   Magbanua Mark Jesus M MJ   Weidner Gerdi G   Weinberg Vivian V   Kemp Colleen C   Green Christopher C   Mattie Michael D MD   Marlin Ruth R   Simko Jeff J   Shinohara Katsuto K   Haqq Christopher M CM   Carroll Peter R PR  

Proceedings of the National Academy of Sciences of the United States of America 20080616 24


Epidemiological and prospective studies indicate that comprehensive lifestyle changes may modify the progression of prostate cancer. However, the molecular mechanisms by which improvements in diet and lifestyle might affect the prostate microenvironment are poorly understood. We conducted a pilot study to examine changes in prostate gene expression in a unique population of men with low-risk prostate cancer who declined immediate surgery, hormonal therapy, or radiation and participated in an int  ...[more]

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