Luminal fructose inhibits rat intestinal sodium-phosphate cotransporter gene expression and phosphate uptake.

ABSTRACT:

Background

While searching by microarray for sugar-responsive genes, we inadvertently discovered that sodium-phosphate cotransporter 2B (NaPi-2b) mRNA concentrations were much lower in fructose-perfused than in glucose-perfused intestines of neonatal rats. Changes in NaPi-2b mRNA abundance by sugars were accompanied by similar changes in NaPi-2b protein abundance and in rates of inorganic phosphate (Pi) uptake.

Objective

We tested the hypothesis that luminal fructose regulates NaPi-2b.

Design

We perfused into the intestine fructose, glucose, and nonmetabolizable or poorly transported glucose analogs as well as phlorizin.

Results

NaPi-2b mRNA concentrations and Pi uptake rates in fructose-perfused intestines were approximately 30% of those in glucose and its ana

SUBMITTER: Kirchner S 

PROVIDER: S-EPMC2430509 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature