MALDI tissue profiling of integral membrane proteins from ocular tissues.

ABSTRACT: MALDI tissue profiling and imaging have become valuable tools for rapid, direct analysis of tissues to investigate spatial distributions of proteins, potentially leading to an enhanced understanding of the molecular basis of disease. Sample preparation methods developed to date for these techniques produce protein expression profiles from predominantly hydrophilic, soluble proteins. The ability to obtain information about the spatial distribution of integral membrane proteins is critical to more fully understand their role in physiological processes, including transport, adhesion, and signaling. In this article, a sample preparation method for direct tissue profiling of integral membrane proteins is presented. Spatially resolved profiles for the abundant lens membrane proteins aquaporin 0

SUBMITTER: Thibault DB 

PROVIDER: S-EPMC2430993 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature