Exon and junction microarrays detect widespread mouse strain- and sex-bias expression differences.

ABSTRACT:

Background

Studies have shown that genetic and sex differences strongly influence gene expression in mice. Given the diversity and complexity of transcripts produced by alternative splicing, we sought to use microarrays to establish the extent of variation found in mouse strains and genders. Here, we surveyed the effect of strain and sex on liver gene and exon expression using male and female mice from three different inbred strains.

Results

71 liver RNA samples from three mouse strains - DBA/2J, C57BL/6J and C3H/HeJ - were profiled using a custom-designed microarray monitoring exon and exon-junction expression of 1,020 genes representing 9,406 exons. Gene expression was calculated via two different methods, using the 3'-most exon probe ("3' gene expression profiling") and u

SUBMITTER: Su WL 

PROVIDER: S-EPMC2432077 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature