RAS signaling in colorectal carcinomas through alteration of RAS, RAF, NF1, and/or RASSF1A.

ABSTRACT: More than half of all colorectal carcinomas are known to exhibit an activated mitogen-activated protein kinase pathway. The NF1 gene, a negative regulator of KRAS, has not previously been examined in a series of colorectal cancer. In the present study, primary colorectal carcinomas stratified according to microsatellite instability status were analyzed. The whole coding region of NF1 was analyzed for mutations using denaturing high-performance liquid chromatography and sequencing, and the copy number alterations of NF1 were examined using multiple ligation-dependent probe amplification and real-time polymerase chain reaction. The mutational hot spots in KRAS and BRAF were sequenced, and promoter hypermethylation status of RASSF1A was assessed with a methylation-specific polymerase chain re

SUBMITTER: Ahlquist T 

PROVIDER: S-EPMC2434205 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature