Exploiting orthologue diversity for systematic detection of gain-of-function phenotypes.

ABSTRACT:

Background

Systematic search for genes whose gain-of-function by exogenous expression confers an advantage in cell-based selective screenings is a powerful method for unbiased functional exploration of the genome, and has the potential to disclose new targets for cancer therapy. A major limit of this approach resides in the labor-intensive cloning of resistant cells, identification of the integrated genes and validation of their ability to confer a selective advantage. Moreover, the selection has to be drastic and genes conferring a limited advantage are typically missed.

Results

We developed a new functional screening strategy based on transduction of mammalian cells of a given species with an expression library from another species, followed by one-shot quantitative tracin

SUBMITTER: Martelli ML 

PROVIDER: S-EPMC2435555 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature