Thrombin-promoted release of UDP-glucose from human astrocytoma cells.

ABSTRACT:

Background and purpose

The P2Y14 receptor is activated by UDP-sugars, most potently by UDP-glucose, but not by free nucleotides, suggesting that UDP-glucose is the cognate agonist for this receptor. However, evidence for regulated release of UDP-glucose is scarce. In the present study, the occurrence of receptor-promoted release of UDP-glucose was investigated, using 1321N1 human astrocytoma cells.

Experimental approach

UDP-glucose release and hydrolysis were measured using HPLC-based techniques. Phospholipase C activation and actin cytoskeleton reorganization were assessed by measuring inositol phosphate formation and fluorescence confocal microscopy, respectively.

Key results

Thrombin and the protease-activating receptor-1 (PAR1) peptide TFLLRNPNDK (PAR1-AP) evoked

SUBMITTER: Kreda SM 

PROVIDER: S-EPMC2437904 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature