A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae.

ABSTRACT: Defined mutant libraries allow for efficient genome-scale screening and provide a convenient collection of mutations in almost any nonessential gene of interest. Here, we present a near-saturating transposon insertion library in Vibrio cholerae strain C6706, a clinical isolate belonging to the O1 El Tor biotype responsible for the current cholera pandemic. Automated sequencing analysis of 23,312 mutants allowed us to build a 3,156-member subset library containing a representative insertion in every disrupted ORF. Because uncharacterized mutations that affect motility have shown utility in attenuating V. cholerae live vaccines, we used this genome-wide subset library to define all genes required for motility and to further assess the accuracy and purity of the library. In this screen, we id

SUBMITTER: Cameron DE 

PROVIDER: S-EPMC2438431 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature