Targeted disruption of the PME-1 gene causes loss of demethylated PP2A and perinatal lethality in mice.

ABSTRACT:

Background

Phosphoprotein phosphatase 2A (PP2A), a major serine-threonine protein phosphatase in eukaryotes, is an oligomeric protein comprised of structural (A) and catalytic (C) subunits to which a variable regulatory subunit (B) can associate. The C subunit contains a methyl ester post-translational modification on its C-terminal leucine residue, which is removed by a specific methylesterase (PME-1). Methylesterification is thought to control the binding of different B subunits to AC dimers, but little is known about its physiological significance in vivo.

Methodology/principal findings

Here, we show that targeted disruption of the PME-1 gene causes perinatal lethality in mice, a phenotype that correlates with a virtually complete loss of the demethylated form of PP2A in

SUBMITTER: Ortega-Gutierrez S 

PROVIDER: S-EPMC2438471 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature