Oxidative stress and COX cause hyper-responsiveness in vascular smooth muscle of the femoral artery from diabetic rats.

ABSTRACT:

Background and purpose

To investigate the dysfunction of vascular smooth muscle in streptozotocin-induced diabetic rats.

Experimental approach

Rings without endothelium of femoral arteries were suspended in organ chambers for isometric tension recording. The production of oxygen-derived free radicals was measured with 2',7'-dichlorodihydrofluorescein diacetate using confocal microscopy. The protein expressions were measured by western blotting.

Key results

The concentration-response curves to U46619 and phenylephrine, but not that to KCl, were shifted to the left, suggesting a hypersensitivity of cell membrane receptors in diabetes. Exogenous oxygen-derived free radicals induced greater vasoconstrictions in the femoral artery from diabetic rats. Chronic treatment with

SUBMITTER: Shi Y 

PROVIDER: S-EPMC2439510 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature