Functional characterization of endogenous siRNA target genes in Caenorhabditis elegans.

ABSTRACT:

Background

Small interfering RNA (siRNA) molecules mediate sequence specific silencing in RNA interference (RNAi), a gene regulatory phenomenon observed in almost all organisms. Large scale sequencing of small RNA libraries obtained from C. elegans has revealed that a broad spectrum of siRNAs is endogenously transcribed from genomic sequences. The biological role and molecular diversity of C. elegans endogenous siRNA (endo-siRNA) molecules, nonetheless, remain poorly understood. In order to gain insight into their biological function, we annotated two large libraries of endo-siRNA sequences, identified their cognate targets, and performed gene ontology analysis to identify enriched functional categories.

Results

Systematic trends in categorization of target genes according t

SUBMITTER: Asikainen S 

PROVIDER: S-EPMC2440555 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature