Gene expression analysis of embryonic stem cells expressing VE-cadherin (CD144) during endothelial differentiation.

ABSTRACT:

Background

Endothelial differentiation occurs during normal vascular development in the developing embryo. This process is recapitulated in the adult when endothelial progenitor cells are generated in the bone marrow and can contribute to vascular repair or angiogenesis at sites of vascular injury or ischemia. The molecular mechanisms of endothelial differentiation remain incompletely understood. Novel approaches are needed to identify the factors that regulate endothelial differentiation.

Methods

Mouse embryonic stem (ES) cells were used to further define the molecular mechanisms of endothelial differentiation. By flow cytometry a population of VEGF-R2 positive cells was identified as early as 2.5 days after differentiation of ES cells, and a subset of VEGF-R2+ cells, that

SUBMITTER: Nikolova-Krstevski V 

PROVIDER: S-EPMC2440556 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature