Temporal profiling of the chromatin proteome reveals system-wide responses to replication inhibition.

ABSTRACT: Although the replication, expression, and maintenance of DNA are well-studied processes, the way that they are coordinated is poorly understood. Here, we report an analysis of the changing association of proteins with chromatin (the chromatin proteome) during progression through interphase of the cell cycle. Sperm nuclei were incubated in Xenopus egg extracts, and chromatin-associated proteins were analyzed by mass spectrometry at different times. Approximately 75% of the proteins varied in abundance on chromatin by more than 15%, suggesting that the chromatin proteome is highly dynamic. Proteins were then assigned to one of 12 different clusters on the basis of their pattern of chromatin association. Each cluster contained functional groups of proteins involved in different nuclear proces

SUBMITTER: Khoudoli GA 

PROVIDER: S-EPMC2440559 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature