Immortalization and characterization of lineage-restricted neuronal progenitor cells derived from the porcine olfactory bulb.

ABSTRACT: Crucial aspects in the development of in vitro neuropathogenic disease model systems are the identification, characterization and continuous mitotic expansion of cultured neuronal cells. To facilitate long-term cultivation, we immortalized porcine olfactory neuronally restricted progenitor cells by genomic insertion of a cDNA encoding the catalytic subunit of the human telomerase reverse transcriptase (hTERT) yielding a stable neuroblast subclone (OBGF400). The altered cells exhibited progenitor-cell-like morphology and mitotic competency based on sustained subpassaging, prevalence in the cell cycle G0/G1 phase and an overall lack of cellular senescence as compared to primary cultures. An OBGF400 neuronal phenotype was indicated by the recognition of a transfected neuronal progenitor-cell-

SUBMITTER: Uebing-Czipura AU 

PROVIDER: S-EPMC2440644 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature