Rapid preparation of nuclei-depleted detergent-resistant membrane fractions suitable for proteomics analysis.

ABSTRACT:

Background

Cholesterol-rich membrane microdomains known as lipid rafts have been implicated in diverse physiologic processes including lipid transport and signal transduction. Lipid rafts were originally defined as detergent-resistant membranes (DRMs) due to their relative insolubility in cold non-ionic detergents. Recent findings suggest that, although DRMs are not equivalent to lipid rafts, the presence of a given protein within DRMs strongly suggests its potential for raft association in vivo. Therefore, isolation of DRMs represents a useful starting point for biochemical analysis of lipid rafts. The physicochemical properties of DRMs present unique challenges to analysis of their protein composition. Existing methods of isolating DRM-enriched fractions involve flotation of cell

SUBMITTER: Adam RM 

PROVIDER: S-EPMC2440737 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature